Journal: Cell Proliferation

Article Title: Narciclasine induces autophagy‐dependent apoptosis in triple‐negative breast cancer cells by regulating the AMPK‐ULK1 axis

doi: 10.1111/cpr.12518

Figure Lengend Snippet: (A) Chemical structure of narciclasine; (B) Cytotoxicity of narciclasine against seven breast cancer cell lines based on the MTT assay: MCF‐7, MDA‐MB‐231, MDA‐MB‐468, BT‐483, BT‐549, HCC‐1937, and MCF‐10A. Results are mean ± SD (N = 3); (C) Narciclasine‐induced autophagy in MDA‐MB‐231 and HCC1937 TNBC cells. All cells were infected with adenovirus‐encoding GFP‐LC3, and the percentage of cells showing fluorescent LC3 puncta (indicative of autophagosomes) was determined. Results are mean ± SD (N = 3); (D) Transmission electron microscopy of autophagy vacuoles; (E‐F) Western blotting of p62, Beclin‐1, and LC3 in total lysates of HCC1937 cells after the indicated treatments

Article Snippet: Cell lines and animals The following breast mammary cell and breast cancer cell lines were obtained from the American Type Culture Collection and cultured in the State Key Laboratory of Biotherapy at West China Hospital of Sichuan University: MCF‐10A, MCF‐7, MDA‐MB‐231, MDA‐MB‐468, BT‐483, BT‐549, and HCC1937.

Techniques: MTT Assay, Infection, Transmission Assay, Electron Microscopy, Western Blot